Day 1 :
Keynote Forum
Robert C DiLuccio
BioChemical Engineering Leader University of Virginia USA
Keynote: Soft tissue augmentation with novel synthetic polymers
Time : 10:00-10:45
Biography:
Experienced Manager/Scientist in leading development of new materials and formulations within the chemical, polymer, consumer, medical device and pharmaceutical industries.
Abstract:
Along with a trend toward living a longer, healthier life, more and more patients are looking to physicians to help them achieve a more youthful appearance. The skin's natural aging process manifests contour changes and rhytids secondary to the depletion of subcutaneous fat and the loss of dermal collagen. Traditionally, rejuvenation has been achieved with a face-lift by surgically tightening the skin. Today, a multitude of minimally invasive procedures are aimed at rejuvenation without the risk, recovery time, and expense of major surgery. The development and popularity of BOTOX RTM has opened the door for equally noninvasive, adjunctive treatment of dynamic rhytids and soft tissue augmentation. Soft tissue augmentation has become a popular means of addressing issues such as, contour defects that result from aging, photo-damage, trauma and/or scarification, or disease. A number of filling agents exist in the armamentarium. Therefore, the physician is responsible for knowing which substance is best suited to address a particular defect and the patient or disease process. rnThis presentation will address compositions and methods of use of novel polymeric materials for soft tissue augmentation and drug delivery. An exemplary composition for augmenting soft tissue can include an active ingredient, a carrier, and a cross-linking agent, wherein the carrier is cross-linked where the cross-linking agent and the active ingredient is combined with the cross-linked carrier into a combination having a therapeutic effect. Examples of current and future products will be discussed for this evolving technology that can lead to solutions for tissue regeneration.rn
Keynote Forum
Pablo Gurman
University of Texas at Dallas, USA
Keynote: A New Generation of Medical Devices Based on Biocompatible Ultrananocrystalline (UNCD®) Diamond Coatings.
Time : 11:00-11:45
Biography:
Dr. Gurman received his MD from Buenos Aires School of Medicine and has been involved in R&D on micro and nanotechnology for medical applications for the las 7 years. Dr Gurman is the author of 20 publications, and is the editor of 2 books. Dr. Gurman holds one patent and has 2 patent pending.
Abstract:
UNCD films co-developed and patented by O. Auciello and colleagues are synthesized by novel microwave plasma chemical vapor deposition and hot filament chemical vapor deposition techniques using an novel patented Ar-rich/CH4 chemistry that produces films with 2-5 nm grains, thus the name UNCD to distinguish them from nanocrystalline diamond films with 30-100 nm grains. The UNCD films exhibit a unique combination of outstanding mechanical, trtibological, electrical, thermal, and biological properties, which already resulted in industrial components and devices currently commercialized by Advanced Diamond Technologies (company co-founded by Auciello and colleagues in 2003).rnThis talk will focus mainly on the application of UNCD coatings for new generation of medical devices, namely: rnrna) Coating for a microchip implantable in the human retina to restore vision to people blinded by retina disorders to improve biocompatibility of silicon microchips used in implantable electronic componentsrnb) Coating for metallic prostheses including dental implants, hips, and knees to practically eliminate body fluids-induced corrosion and biofouling in current failing metal prostheses rnc) Coating of polymeric devices to improve antifouling propertiesrnd) Development of a new generation of BioMEMS/BioNEMS devices based on UNCD to increase tribological and biological performancerne) Development of piezoelectric multilayer MEMS devices based on biocompatible UNCD/AlN, and UNCD/BiFeO for implantable biosensors to replace piezoactuated BioMEMS based on biologically non-compatible Lead-based piezoelectric PZT rnrnThe biocompatible UNCD coatings are being developed for commercialization in a new generation of implantable medical devices, through a startup company (Original Biomedical Implants (OBI)) co-founded by Auciello and Gurman in 2013.rn
- Track 1: Clinical and Diagnostic Biomedical Devices Track 4: Biosensors and Bioelectronics Track 5: Rehabilitation Engineering and Surgical Devices Track 6: Applications of Biomedical Engineering Track 7: Nano materials and Nanotechnology Track 8: Research On Biomedical Imaging and Bio signals Track 9: Diagnostic Techniques in Dermatology and Cosmetic Dermatology
Chair
Dr.Pablo Gurman
University of Texas at Dallas
Session Introduction
Sandra N Osborne
KCI- an Acelity Company, USA
Title: An automated and minimally invasive tool harvests viable epidermis capable of cellular outgrowth using a bench skin model
Time : 11:55-12:25
Biography:
Sandra N Osborne is a tissue culture specialist in the Department of Device Sciences, headquartered in San Antonio’s KCI R&D facility. She obtained a PhD from the University of California at San Diego, and has over 20 years of experience as a research scientist. Her published record covers topics in epigenetic silencing and cellular response in organisms ranging from yeast to humans. During her tenure at KCI, she has executed research that elucidated mechanisms of action for a number of products including: CelluTome™, an epidermal micrografting tool; Graftjacket®, an acellular dermal matrix and V.A.C® on multiple patient stem cell populations.
Abstract:
An epidermal harvesting tool that makes epidermal grafting possible in an outpatient setting with minimal donor site damage has recently been described. The technique involves a device that applies both heat and suction concurrently to normal skin over time to induce epidermal graft formation. In order to investigate the biological mechanims of epidermal graft formation and transfer, a bench model for creating and harvesting grafts was developed. In this study, detached human abdominoplasty or cadaveric skin was used as a model of epidermal graft formation and adhesion. Graft adhesion was assessed using an open-mesh silicone dressing and a transparent film dressing to transfer grafts obtained from various anatomical locations. The study results showed that epidermal grafts were created after approximately 18-75 minutes on, both, unfrozen and previously frozen cadaveric skin that was stretched onto a supporting surface. Different anatomical locations showed variable graft formation times. The grafts were tested for viability and results showed that detached epidermal skin obtained 24 hr post-mortem was viable, but lost viability when obtained 48 hr post-mortem. Abdominoplasty skin was viable and capable of cellular outgrowth in culture. These results showed that cadaveric skin, although not a suitable model for testing epidermal cell outgrowth after harvest, is a suitable model for studying epidermal graft formation and harvesting techniques using various advanced approaches.
Alicja E Kownacka
University of Hull, UK
Title: Synthesis of MRI contrast agents for the non invasive imaging and evaluation of tissue engineering constructs
Time : 12:25-12:55
Biography:
Alicja E Kownacka was awarded MSc in Biomaterials degree from Westpomeranian University of Technology, Poland, where she worked on photo curable polymeric systems for novel hernia treatment. For the following year, she worked in 3 different European Institutes developing nanomaterials for various applications including MR imaging. In 2014, she won a Marie-Curie PhD scholarship and joined Prof. Steve Archibald’s group at University of Hull, UK, where she is currently working on a non-invasive visualisation of soft and hard tissue engineering constructs using Magnetic Resonance Imaging (MRI) and Positron Emission Tomography (PET).
Abstract:
Visualisation of tissue engineering constructs in vivo is required in order to monitor their behaviour and integration into surrounding tissues or to promptly detect immune rejection. Various analytical tools could be used to that end, however, most of them require invasive biopsies and allow only a single time point characterisation. Magnetic resonance imaging (MRI) is a non-invasive technique, therefore, its potential usage in tissue engineering area is a major focus of research effort. Nevertheless, MRI suffers from poor sensitivity, thus, there is a clear need for the development of novel contrast agents and also for elaboration of methodologies allowing their conjugation to the engineered materials. Presently, biomedical hydrogels used for soft tissue reconstructions cannot be distinguished from a normal tissue by MRI due to their high water content. Similarly, calcium phosphate cements (CPCs) designed for bone regeneration are too dense and do not contain the required amount of water for MR imaging. These limitations can be overcome by use of stable metal chelates or nanomaterials as contrast agents. Current work on gadolinium complexes functionalised for chemospecific conjugation with polyisocyanopeptide hydrogels and development of bone targeted gadolinium nanoparticles will be presented.
Kenneth P Grismore
Xacobo Bio-Engineering, USA
Title: Synthetic Blood – Artificially grown red blood cells
Time : 14:00-14:20
Biography:
Kenneth P Grismore completed his PhD from University of South Carolina. He is the CEO of Xacobo Bio-Engineering. He was scout and a front line medic in the US Army. Worked for 5 years at MUSC as a Certified Anesthesia Technician and Supply Specialist. Proficient in all aspects of anesthesia and warehouse logistics. He is currently working on a classified project that will revolutionize the blood product industry.
Abstract:
Blood donations are one day a thing of the past; thanks to the creation of the first functional red blood cells grown in the lab. Embryonic stem cells (ESCS) are the origin of these cells. The breakthrough raises the prospect of producing mass supplies of the “Universal Donor” blood type O-negative, which is highly desirable because it can be safely transferred into patient, irrespective of their blood group. This type of blood is in short supply-of around Caucasians with 8% and just 0.3% of Asians. Instead of getting blood from countless donors making it from a few ESC lines may also help to stop the spread of disease, as it is easier to ensure such artificial blood is free of pathogens such as HIV and the viruses that cause hepatitis. To create the red blood cells, the cells were exposed to cultures of human ESCS to a sequence of nutrients and growth factors. This turned them to be named as Haemangioblasts, at the beginning which are precursors to blood cells, and then into mature red blood cells.
Sen Hou
Polish Academy of Sciences, Poland
Title: Measure the DNA double labeling efficiency by fluorescence correlation spectroscopy
Time : 14:20-14:50
Biography:
Sen Hou has completed his PhD from Nankai University, China in 2009 and then become an Assistant Professor in Institute of Physical Chemistry, Polish Academy of Sciences Poland. He has published more than 40 papers in reputed journals and has been serving as a regular reviewer for repute journals. He is a Biologist and is active in the cross-field of Chemistry and Physics.
Abstract:
Double-stranded DNA (dsDNA), fabricated by annealing of complementary single-stranded DNA (ssDNA) labeled with the same fluorescent dyes results in the coexistence of dsDNA molecules labeled with one or two dyes. Existing methods are insufficient to measure the percentage of the doubly-labeled dsDNA component in the fluorescent DNA sample and cannot even distinguish the doubly-labeled DNA component from the singly-labeled component. We presented a method based on fluorescence correlation spectroscopy (FCS) technique to measure the percentage of doubly-labeled DNA. In each method, we added known amount of unlabeled and imperfect labeled ssDNA and perform annealing on such DNA samples to obtain dsDNA. Next we measured the FCS autocorrelation function of the resulting dsDNA samples. After we fitted amplitudes and triplet state proportion from the FCS autocorrelation curves, we were able to calculate the percentage of doubly-labeled DNA in the total fluorescent component, which is defined as DNA double labeling efficiency (DDLE). We evaluate the precision of our method by the amplification of coefficient variation from the original experiment data to the measured double labeling efficiency. We expressed the amplification of the coefficient variation as a function of labeling efficiency of ssDNA and also as the measured doubly labeling efficiency. We find that the method is more precise with c increasing. The measurements need only femtoliter volume of dsDNA samples of anomolar level of concentrations and cost tens of minutes to complete measurement.
Obinna K. Ihesiulor
University of New South Wales
Title: Investigation of wear in orthopaedic hip prosthetic devices
Time : 14:50-15:20
Biography:
Obinna K. Ihesiulor is presently a PhD student at the University of New South Wales, Canberra, Australia. He obtained his Master’s degree in 2012 at the same university. His current research interest is in the area of prosthetic devices for joint replacements. His PhD research work is on the investigation of wear of total hip replacement at the taper-trunnion junction. In order to quantify the amount of material loss to ultimate locations in the surrounding joint space, the taper-trunnion project for wear investigation is conceptualized and motivated. In a broader sense, the principal goal is to work toward the minimization of wear debris produced in the hip joint, thereby resulting in a longer prosthetic lifetime.
Abstract:
Total hip replacement (THR) is one of the most popularly performed operations in orthopaedics as evident in clinical outcomes. Potentially, an approximate of 400,000 hip joint replacement procedures is performed each year in the U.S. However, studies have identified that majority of hip implants are prone to wear at the taper-trunnion junction. The taper-trunnion (head-stem) is essentially the interface between the ball of the hip joint which articulates with the pelvis and the hip stem which is constrained rigidly to the femur bone. The head and stem components connect at a taper on the hip head and a trunnion on the hip stem. This junction is of peculiar and remarkable interest because it is associated with excessive fretting wear due to the taper sliding relatively against the trunnion. Excessive fretting wear at the taper-trunnion junction (trunnionosis) apparently contributes to the high failure rates of hip implants. Implant wear and corrosion lead to the release of metal particulate debris and subsequent release of metal ions at the taper-trunnion surface. This results in a type of metal poisoning referred to as metallosis. The consequences of metal poisoning include; osteolysis (bone loss), osteoarthritis (pain), aseptic loosening of the prosthesis and revision surgery. Follow up after revision surgery, metal debris particles are commonly found in numerous locations. To investigate the feasibility of reducing wear in taper/head-trunnion/stem (head-stem) joints in hip implants by the use of cylindrical/parallel head-stem joints instead of the currently used tapered stems.
Akintayo Kehinde Akeen
University of Lagos, Nigeria
Title: Operational safety improvement in medical-grade oxygen production plant in some Nigerian hospitals
Time : 15:20-15:50
Biography:
Akintayo Kehinde Akeen is a Master of Process Engineering Degree holder from University of Lagos, Nigeria. He is an American Society of Sanitary Engineer Certified Medical Gas System Specialist. He has over seven years working on Oxygen Production at BOC Gases Nigeria and Federal University Teaching Hospital as Safety Intern/Plant Engineer and Medical Oxygen Plant Safety Engineer. He has presented five (5) Technical Paperson Medical and Industrial gases. He is a Corporate Member Nigerian Society of Chemical Engineer and Nigerian Society of Engineers. He has volunteered his services as Medical Gas System Specialist to some public hospitals in Nigeria to ensure improved services.
Abstract:
Medical-grade oxygen constitutes a source of life in hospitals. Medical oxygen belongs to the medical gases family utilized by medical practitioners in treating sick patient in the hospitals. The methodology adopted for this research work is the administration of questionnaire to the most senior technical personnel in the plant operation hierarchy, while regular visit to the oxygen plant was conducted in the course of this research work. Major findings from this research are the absences of developed and implementable written Safety Information, No established systems to response to plant operational hazards, Process flow diagram of Medical Oxygen Plants are no easily accessible to plant engineers, No evidence of safety meetings or induction, Use of Personnel Protective Equipment (PPE) was not in force, No evidence of Accident or Near misses reporting or documentation nor Plant daily logging system or documented plant operation monitoring system. The major improvement to the Oxygen Plant at the conclusion of this research work is the adoption of an online Oxygen Plant performance daily logging system, adoption of no work without PPE, Safety induction of Plant Operator and other Plant supporting personnel was proposed for adoption, Process flow diagram of the Oxygen Plant was made available to the Plant Operator for understanding of plant flow sheet, Safety Policy sample was made submitted for adoption.
Mohamed M Dessouky
University of Menoufiya, Egypt
Title: Statistical Analysis of 3D Images of Alzheimer’s disease
Time : 16:00-16:30
Biography:
Mohamed M. Dessouky was born in Egypt, 27 April 1984. Graduated from department of Computer Science and Engineering, Faculty of Electronic Engineering, Menoufiya University, Egypt at 2006. Demonstrator at 2007, Assistant Lecturer at 2011. Now he is a PhD student. The major field of study is image processing and artificial intelligence. Mohamed has More than six years of teaching experience as an assistant lecturer, and Teaching Assistant for a variety of undergraduate courses in different Computer science and Engineering fields. Dr. Mohamed is CISCO Certified Instructor and got award from CISCO as a best instructor for more than 5 years
Abstract:
Alzheimer’s disease is the most common type of dementia which it has no cure nor imaging test for it. Diagnosis of the Alzheimer’s disease (AD) still a challenge and difficult. An early diagnosis for Alzheimer’s disease is very important to delay the progression of it. This paper extract and analyze various important features of 3D-MRI brain medical images to provide better analysis and diagnosis of AD. These extracted features had been used for detection of the abnormalities among different demented and non-demented MRI AD images. This paper deals with the statistical analysis to discriminate among the different types of tissue. Also, it investigates and building up an efficient Computer Aided Diagnosis (CAD) system for AD to assist the medical doctors to easily diagnose the disease. Statistical, structural, and textural features had been extracted for different images. These extracted features had been used as an input to the SVM classifier. In addition, all these features had been applied to the proposed algorithm and then had been classified using SVM classifier. The performance of the CAD system based on statistical analysis and the proposed algorithm had been measured using different metric parameters. Also, the proposed algorithm had been applied to the images with intensity level. The obtained results indicate that the metric parameters increase from 60% without using the proposed algorithm to 100% using the proposed algorithm. Alzheimer’s disease is a degenerative brain disease and the most common cause of dementia. The most common initial symptom is a gradually worsening ability to remember new information, planning or solving problems, completing familiar tasks at home or work, Confusion with time or place, and problems with words in speaking or writing. Alzheimer’s disease is a progressive disease, which means that it gets worse over time. There is no cure, specific blood or imaging test for Alzheimer’s disease. However, some drugs are available which may help slow the progression of Alzheimer’s symptoms for a limited time. Diagnosis of the Alzheimer’s disease (AD) still a challenge and difficult, especially in the early stages. The early detection will be key to prevent, slow and stop Alzheimer’s disease. The last 10 years have seen a tremendous growth in research on early detection. Statistical analysis method is one of the important methods for feature extraction in digital images. There are different previous approaches that depends on extracting statistical, textural, and structural features from digital images in different application. The statistical analysis of 3D and 2D images of AD had been presented in this paper. Different important statistical, structural and textural features that had been extracted from different AD MRI images (normal, very mild AD and mild AD). The 3D images had been analyzed in three plans and the features had been extracted from each plane. Studying and analyzing these extracted features may help the medical doctors to diagnose the Alzheimer’s disease.
Namrata Tiwari
Indian Institute of Technology- BHU, India
Title: Photo-luminescent carbon quantum dot-layered double hydroxide composites for diagnostic applications
Time : 16:30-17:00
Biography:
Namrata Tiwari is currently pursuing an Integrated Dual Degree course that incorporates a B-Tech in Bio-engineering and M-Tech in Bio Medical Technology at the School of Biomedical Engineering, Indian Institute of Technology- Banaras Hindu University [IIT-(BHU)], Varanasi, India. She wishes to apply for the poster presentation owing to her experience and interest. She has worked as a research intern in the Defense Institute of Technology (DRDO), Pune under the supervision of “Dr. H S Panda”, Assistant Prof., department of Material Engineering, DIAT, Pune and has contributed in a commendable research work
Abstract:
There is an increased need of materials that can effectively help in the process of cancer diagnosis. Use of luminescent carbon quantum rods in biological investigations has increased dramatically over the past several years due to their unique size dependent optical properties, low cytotoxicity and good biocompatibility. Photo-luminescent carbon based materials, which act as bio label, can be used as fillers in a polymer/inorganic matrix to synthesize a composite material for required application. Layered double hydroxide (LDH) was used as the matrix material as it easily incorporates the bio markers/labels and helps in decreasing the toxic effect the particles might have. We have synthesized a hybrid material consisting of Layered Double Hydroxide (LDH) as matrix and CQRs as filler. CQRs were synthesized using electrochemical method in which graphite rod electrodes were dipped in a basic solution. LDH, which is a multilayered material, was then formed. To further enhance the properties of LDH, CQR was incorporated in-situ in it by co-precipitation method in which we took 10ml of CQD solution in 60 ml of water and adding Mg and Al salt drop wise maintaining the pH at 11 and then heating at 60 degree for 24 hrs. Then,centrifugation was done and the sediment was dried and characterized for obtaining its properties. The composite prepared were characterized by XRD and TEM techniques. The other techniques used were SEM, FTIR, Raman spectroscopy, cell culture, T to T1 relaxation. Further, tests on cytotoxicity have been performed to test the compatibility with living cells.
Sandra N Osborne
Tissue culture specialist in the Department of Device Sciences, headquartered in San Antonio’s KCI R&D facility
Title: An automated and minimally invasive tool harvests viable epidermis capable of cellular outgrowth using a bench skin model
Time : 11:30 - 12:00
Biography:
Dr. Osborne is a tissue culture specialist in the Department of Device Sciences, headquartered in San Antonio’s KCI R&D facility. She obtained a Ph.D. from the University of California at San Diego, and has over 20 years of experience as a research scientist. Her published record covers topics in epigenetic silencing and cellular response in organisms ranging from yeast to humans. During her tenure at KCI, Dr. Osborne has executed research that elucidated mechanisms of action for a number of products including: CelluTome™, an epidermal micrografting tool; Graftjacket®, an acellular dermal matrix; and V.A.C® on multiple patient stem cell populations.
Abstract:
An epidermal harvesting tool* that makes epidermal grafting possible in an outpatient setting with minimal donor site damage has recently been described 1,2. The technique involves a device that applies both heat and suction concurrently to normal skin over time to induce epidermal graft formation. In order to investigate the biological mechanims of epidermal graft formation and transfer, a bench model for creating and harvesting grafts was developed. In this study, detached human abdominoplasty or cadaveric skin was used as a model of epidermal graft formation and adhesion. Graft adhesion was assessed using an open-mesh silicone dressing¥ and a transparent film dressingâ‚´ to transfer grafts obtained from various anatomical locations. The study results showed that epidermal grafts were created after approximately 18-75 minutes on, both, unfrozen and previously frozen cadaveric skin that was stretched onto a supporting surface. Different anatomical locations showed variable graft formation times. The grafts were tested for viability, and results showed that detached epidermal skin obtained 24 hr post-mortem was viable, but lost viability when obtained 48 hr post-mortem. Abdominoplasty skin was viable and capapble of cellular outgrowth in culture. These results showed that cadaveric skin, although not a suitable model for testing epidermal cell outgrowth after harvest, is a suitable model for studying epidermal graft formation and harvesting techniques using various advanced approaches. *CelluTome™ Epidermal Harvesting System (KCI, an Acelity Company, San Antonio, TX), ¥Adaptic Touch™ (Systagenix, an Acelity Company, Gargrave, UK), Tegaderm™ Film Dressing (3M, St. Paul, MN).
Sandra N Osborne
Tissue culture specialist in the Department of Device Sciences, headquartered in San Antonio’s KCI R&D facility
Title: An automated and minimally invasive tool harvests viable epidermis capable of cellular outgrowth using a bench skin model
Time : 11:55-12:25
Biography:
Dr. Osborne is a tissue culture specialist in the Department of Device Sciences, headquartered in San Antonio’s KCI R&D facility. She obtained a Ph.D. from the University of California at San Diego, and has over 20 years of experience as a research scientist. Her published record covers
Abstract:
An epidermal harvesting tool* that makes epidermal grafting possible in an outpatient setting with minimal donor site damage has recently been described 1,2. The technique involves a device that applies both heat and suction concurrently to normal skin over time to induce epidermal graft formation. In order to investigate the biological mechanims of epidermal graft formation and transfer, a bench model for creating and harvesting grafts was developed. In this study, detached human abdominoplasty or cadaveric skin was used as a model of epidermal graft formation and adhesion. Graft adhesion was assessed using an open-mesh silicone dressing¥ and a transparent film dressingâ‚´ to transfer grafts obtained from various anatomical locations. The study results showed that epidermal grafts were created after approximately 18-75 minutes on, both, unfrozen and previously frozen cadaveric skin that was stretched onto a supporting surface. Different anatomical locations showed variable graft formation times. The grafts were tested for viability, and results showed that detached epidermal skin obtained 24 hr post-mortem was viable, but lost viability when obtained 48 hr post-mortem. Abdominoplasty skin was viable and capapble of cellular outgrowth in culture. These results showed that cadaveric skin, although not a suitable model for testing epidermal cell outgrowth after harvest, is a suitable model for studying epidermal graft formation and harvesting techniques using various advanced approaches. *CelluTome™ Epidermal Harvesting System (KCI, an Acelity Company, San Antonio, TX), ¥Adaptic Touch™ (Systagenix, an Acelity Company, Gargrave, UK), Tegaderm™ Film Dressing (3M, St. Paul, MN).